Pertinence of developing highly sensitive test for borrelia spp. 

Phelix is working on setting up a phages based test (blood or/and urine) for borrelia spp. as the current available tests return divergent results.

The clinician can not rely on one secure and sensitive test to diagnose Lyme borreliosis; serological tests are very often false negative and the practitioner has to consider first clinical symptoms.

A reliable test would help to treat undiagnosed or badly diagnosed conditions and pastients.

See the two following articles regarding the diagnosis of lyme borreliosis in Autism.

Divergent Opinions of Proper Lyme Disease Diagnosis in ASD

This paper proposes that some children with an autism spectrum disorder (ASD) in the United States have undiagnosed Lyme disease and different testing criteria used by commercial laboratories may be produc- ing false negative results.


Serologic Markers of Lyme Disease in Children with Autism

A proposed link between Lyme disease and autism has garnered considerable attention. Among individuals with autism spectrum disorders, rates of seropositivity for Lyme disease of greater than 20% have been reported.


See the complixity of avaialble tests and their pertinence 

Diagnosis of Lyme Borreliosis

This review attempts to provide a comprehensive assessment of the development and application of currently available tests for the laboratory diagnosis of LB. Future directions for im- provement of established tests and for development of new approaches are also discussed.


Phelix has set up a new phage based PCR for borrelia spp. that is more than 50% more sensitive than the current available tests.

Experimentally confirmed that our phage-based PCR showed significantly increased sensitivity compared to the traditional 5S-23S intergenic region based PCR (50% higher compared to other borrelia tests). As seen in the gel picture below, only one positive (IV) using bacteria-based PCR, while two positives can be identified using phage-based PCR 

Also developed a Borrelia 16S nested PCR, which involved a bacterial universal PCR followed by a Borrelia PCR. This PCR will pick up all Lyme Borrelia strains.